![]() ![]() ![]() LC-MS, on the other hand, generally requires sample preparation, chromatographic separation, specific experimental and ionization conditions, instrumentation and operator skill. These make it difficult to standardize MS analysis. ![]() In contrast, NMR does not require elaborate sample preparation and fractionation, is highly reproducible, and is able to provide both qualitative and quantitative information on chemically diverse compounds. The standardization of the NMR protocol will further improve the usefulness of NMR as a tool for the profiling of natural products extracts. Because NMR is able to detect compounds only down to 0.1% level, it is not suitable for the detection of trace components. NMR is less sensitive than MS, which can detect compounds down to parts per million (ppm) levels. Because of the distinct advantages of each method, NMR and MS are considered as complementary techniques. NMR is a quantitative spectroscopic tool because the intensity of the peaks is directly proportional to the number of nuclei. ![]()
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